TABLE 2.

Subcellular distribution of HA-Nrf2^a

Keap1 proteinb	% Subcellular distribution inc:
	N	N-C	C
Noned	0	100	0
WT	0	0	100
ΔN	0	0	100
ΔBTB	0	0	100
ΔL	50	50	0
ΔK	0	100	0
ΔC	0	0	100

^aThe subcellular localization of HA-Nrf2 in NIH 3T3 cells was determined by indirect immunofluoresence.

^bThe indicated Keap1 proteins were coexpressed with Nrf2 in NIH 3T3 cells. WT, wild type.

^cPercentages of cells that displayed predominantly nuclear (N), nuclear/cytoplasmic (N-C), and cytoplasmic (C) staining of Nrf2 are presented. A total of 100 cells that are positive for expression of Nrf2 and the indicated Keap1 protein were examined.

^dHA-Nrf2 was expressed alone (without a Keap1 protein) in NIH 3T3 cells.