TABLE 2.
Mating efficiency of mfα and ste2 mutants of C. albicansa
| Strain (Ura−) | Mating type | Relevant genotype | Mating efficiency
|
|
|---|---|---|---|---|
| MMY278 a (Ade−) | CHY477 α (Ade−) | |||
| CHY439 | a | Wild type | <10−8 | 2.3 × 10−1 |
| CHY257 | α | Wild type | 4.8 × 10−1 | 3.6 × 10−7 |
| MMY564 | a | mfα/mfα | <10−8 | 5.0 × 10−2 |
| MMY563 | α | mfα/mfα | <10−8 | <10−8 |
| MMY566 | a | ste2/ste2 | <10−8 | <10−8 |
| MMY565 | α | ste2/ste2 | 1.8 × 10−1 | <10−8 |
| MMY567 | a | ste2/ste2 | <10−8 | <10−8 |
| MMY568 | α | ste2/ste2 | 1.6 × 10−1 | 5.4 × 10−8 |
a
Gene knockouts of MFα and STE2 were created, and opaque a and α derivatives (Ura−) mated with opaque control mating strains (Ade−). The frequency of mating was determined by the number of colonies that grew on Ura− Ade− medium, as described in Materials and Methods.