TABLE 3.
Genes whose transcription is induced more than three-fold in a opaque cells in response to α-factora
| Geneb | ORF6 designationc | Fold inductiond | S. cerevisiae homologe | Induced in S. cerevisiaef | Filamentationg | Functionh |
|---|---|---|---|---|---|---|
| ORF6.7473 | ORF6.7473 | 104 | YPS3 | No | Weak similarity to GPI-anchored aspartyl protease | |
| YMR244w | ORF6.9141 | 73 | YMR244W | No | Unknown | |
| PRM1 | ORF6.6562 | 57 | PRM1 | Yes | Plasma membrane fusion | |
| FUS1 | ORF6.3131 | 51 | FUS1 | Yes | Required for cell fusion in mating | |
| ORF6.3434 | ORF6.3434 | 46 | Putative lectin binding domain | |||
| FIG1 | ORF6.3476 | 41 | FIG1 | Yes | Involved in cell polarization and cell fusion | |
| POL/TCA3 | ORF6.1312 | 39 | Reverse transcriptase | |||
| ORF6.4462 | ORF6.4462 | 38 | Unknown | |||
| FGR23 | ORF6.1030 | 37 | Yes | Extracellular α-1,4-glucan | ||
| HST6 | ORF6.8600 | 33 | STE6 | Yes | Transporter of a-factor | |
| SST2 | ORF6.2767 | 25 | SST2 | Yes | Adaptation to pheromone | |
| RBT1 | ORF6.2929 | 25 | Yes | TUP1-regulated, HWP1-related cell wall protein | ||
| HWP1 | ORF6.4883 | 22 | FLO11 | No | Yes | GPI-anchored aspartyl protease |
| CEK2 | ORF6.2854 | 15 | FUS3 | Yes | MAP kinase in mating cascade | |
| SAP4 | ORF6.3803 | 15 | YPS3 | No | Yes | Secreted aspartyl protease |
| STE2 | ORF6.4012 | 13 | STE2 | Yes | α-Factor pheromone receptor | |
| RBT4 | ORF6.538 | 12 | PRY3 | No | Yes | TUP1-regulated, similar to plant pathogenesis proteins |
| ORF6.5658 | ORF6.5658 | 11 | YOL157C | Yes | Unknown | |
| SAP6 | ORF6.3624 | 11 | YPS3 | No | Yes | Secreted aspartyl protease |
| GAG | ORF6.3764 | 10 | Retrotransposon | |||
| KAR4 | ORF6.1096 | 9 | KAR4 | Yes | Karyogamy | |
| ORF6.4265 | ORF6.4265 | 9 | Unknown | |||
| SAP5 | ORF6.4427 | 9 | YPS3 | No | Yes | Secreted aspartyl protease |
| SOK1 | ORF6.3088 | 9 | SOK1 | No | Unknown | |
| ORF6.333 | ORF6.333 | 8 | Leucine-rich repeat family protein | |||
| ECE1 | ORF6.2886 | 8 | Yes | Filament-specific cell wall protein | ||
| ORF6.1965 | ORF6.1965 | 7 | Weak similarity to bacterial α-1,6-mannanase | |||
| ORF6.1768 | ORF6.1768 | 7 | Unknown | |||
| RAM1 | ORF6.3323 | 7 | RAM1 | No | Farnesyltransferase that prenylates a-factor | |
| CEK1/ERK1 | ORF6.1819 | 7 | KSS1 | No | Yes | MAP kinase |
| DEM1 | ORF6.6315 | 7 | DEM1 | No | Unknown, weak similarity to pre-tRNA processing | |
| ORF6.5533 | ORF6.5533 | 6 | Unknown | |||
| MOH1 | ORF6.2891 | 6 | MOH1 | No | Unknown | |
| ORF6.5572 | ORF6.5572 | 6 | PRY3 | No | Similarity to plant pathogenesis-related proteins | |
| ORF6.8898 | ORF6.8898 | 6 | Leucine-rich repeat family | |||
| ORF6.2507 | ORF6.2507 | 6 | Unknown | |||
| GAS1 | ORF6.3873 | 6 | GAS1 | No | 1,3-β-glucanosyltransferase activity | |
| ORF6.5495 | ORF6.5495 | 6 | Unknown | |||
| ORF6.3472 | ORF6.3472 | 5 | Unknown | |||
| ORF6.2403 | ORF6.2403 | 5 | YIL130W | No | Unknown, similar to transcription factors | |
| EXG1 | ORF6.1982 | 5 | EXG1 | No | Glucan 1,3-β-glucosidase activity | |
| YDR124W | ORF6.8915 | 5 | YDR124W | Yes | Unknown | |
| CPH1 | ORF6.695 | 5 | STE12 | Yes | Yes | Transcription factor |
| RAM2 | ORF6.5223 | 5 | RAM2 | No | Farnesyltransferase that prenylates a-factor | |
| ORF6.4292 | ORF6.4292 | 5 | Unknown, homoserine kinase motif | |||
| RHR2 | ORF6.8673 | 5 | RHR2 | No | Glycerol-1-phosphatase activity, response to osmotic stress | |
| KAR5 | ORF6.2573 | 5 | KAR5 | Yes | Component of cytoskeleton | |
| ORF6.4127 | ORF6.4127 | 4 | MNN4 | No | N-linked glycosylation | |
| YKR043C | ORF6.4339 | 4 | YKR043C | No | Unknown | |
| PCL1 | ORF6.1258 | 4 | PCL1 | Yes for PCL2 | G1/S-specific cyclin/PICK> | |
| ORF6.3431 | ORF6.3431 | 4 | Unknown | |||
| ORF6.5360 | ORF6.5360 | 4 | Unknown | |||
| ORF6.3939 | ORF6.3939 | 4 | Unknown | |||
| AXL1 | ORF6.8423 | 4 | AXL1 | Yes | Bud site selection, pheromone maturation | |
| RSN1 | ORF6.5211 | 4 | RSN1 | No | Unknown | |
| ORF6.8300 | ORF6.8300 | 4 | Unknown | |||
| KAR9 | ORF6.7998 | 3 | KAR9 | No | Karyogamy | |
| MUM2 | ORF6.4410 | 3 | MUM2 | No | Premeiotic DNA synthesis | |
| CPP1 | ORF6.7345 | 3 | MSG5 | Yes | Adaptation to pheromone | |
| ORF6.5308 | ORF6.5308 | 3 | YHB1 | No | Probable flavohemoprotein, response to stress | |
| ORF6.8381 | ORF6.8381 | 3 | Unknown | |||
| ORF6.8384 | ORF6.8384 | 3 | Unknown |
None of these genes is induced in α opaque cells when treated with α-factor. Gene name, if assigned. If not assigned, gene name based on the closest S. cerevisiae homolog. Identification number assigned by the Stanford genome project. These identification numbers are based on assembly 6 available at www-sequence.stanford.edu/group/candida. Fold induction by α-factor. If a closely related S. cerevisiae gene exists (Blast score of at least 10−6), its name is given in this column. Induced in S. cerevisiae a cells in response to S. cerevisiae α-factor (35). Implicated in filamentous growth and/or virulence. Two of the genes implicated in filamentous growth (ORF6.3472 and ORF6.4292) were identified in microarray experiments (D. Kadosh and A. D. Johnson, unpublished observations). FGR23 was recently shown to be defective in filament formation (40). A brief synopsis of the known or inferred role of the indicated gene.