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Spt16 association occurs just downstream of promoter. (A) Regions of the GAL1-YLR454 gene used to map protein occupancy at the promoter-proximal region. (B) Chromatin from galactose-induced cells containing either Hpr1-(Myc)13, HA-Rpb3, Spt16-(Myc)3, TBP, or Tfg2-(Myc)3 was immunoprecipitated with appropriate antibodies and analyzed by quantitative PCR. (C) Relative levels of Spt16 and Hpr1 with respect to Pol II at the indicated regions.
