TABLE 2.

Determinations of pre-steady-state kinetics parameters for correct and incorrect nucleotide incorporation by human DNA Polη, yeast DNA Polη, and the Klenow fragment of E. coli DNA polymerase I

Polymerase	Results
	KDdNTP (μM)	kpol (s−1)	kpol/KDdNTP	KDdNTPincorrect/KDdNTPcorrect	kpolcorrect/kpolincorrect	Fidelitya
Human Polη
Correct	110	67	0.61
Incorrect	380	0.77	2.0 × 10−3	3.5	87	300
Yeast Polηb
Correct	2.4	1.3	0.54
Incorrect	13	8.7 × 10−3	6.7 × 10−4	5.4	150	810
E. coli Klenow fragmentc
Correct	5	50	10
Incorrect	17	1.0 × 10−2	5.9 × 10−4	3.4	5,000	17,000

^aFidelity was calculated by the following equation: (k_pol/K_D^dNTP)_correct/(k_pol/K_D^dNTP)_incorrect.

^bData are from Washington et al. (24).

^cData are from Kuchta et al. (12, 13).