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Concentration of ganglioside GM1 and H-ras in CLDs. Equal amounts of proteins from CLDs, plasma membranes, postnuclear supernatants, and lysates of ScN2a and N2a cells were probed with cholera toxin B conjugated to horseradish peroxidase (lanes 1–8) and a mAb against H-ras (lanes 9–16).
