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PrPC and PrPSc are concentrated in CLDs on the surface of ScN2a cells. Detection of streptavidin-agarose-precipitated PrP from CLDs derived from biotinylated plasma membranes of N2a and ScN2a cells isolated with the detergent-free method. Immunoblots of CLD proteins precipitated with streptavidin-agarose (lanes P) and unbound proteins of the respective supernatants (lanes S) using anti-PrP R073 antiserum without (− PK) or with (+ PK) limited digestion by proteinase K.
