FIG. 4.

DTRAF1 induces activation of the JNK signaling pathway and apoptosis. (A and B) The eye imaginal discs were immunostained with an anti-phospho-specific JNK antibody as described in Materials and Methods. (A) gmr-GAL4/+. (B) gmr-GAL4, EP(2)0578/+. (C and D) puckered-LacZ reporter assays were also conducted in the eye discs. (C) gmr-GAL4/+; puckered-LacZ/+. (D) gmr-GAL4, EP(2)0578/+; puckered-LacZ/+. (E to H) DTRAF1-induced apoptosis in the eye discs was examined by TUNEL assays. (E) gmr-GAL4/+. (F) gmr-GAL4, EP(2)0578/+. (G) gmr-GAL4, EP(2)0578/EP(2)0578. (H) hep¹/Y; gmr-GAL4, EP(2)0578/+.