FIG. 7.

DTRAF2 overexpression induces antimicrobial gene expression in situ. Third-instar larvae of the designated genotypes were either infected with Escherichia coli or heat shocked at 37°C for 3 h as described in Materials and Methods. (A to D) The larvae were examined under a fluorescence microscope to locate the GFP-expressing tissues. (E to H) In addition, the fat bodies dissected from the larvae were examined by using a fluorescence microscope. (I to L) In the case of diptericin-LacZ reporter larvae, the fat bodies were X-Gal stained and observed under light microscope. (A, B, E, and F) drosomycin-GFP/X; hs-GAL4/+. (C and G) drosomycin-GFP/X; hs-GAL4/EP(2)0578. (D and H) EP(X)1516/drosomycin-GFP; hs-GAL4/+. (I and J) diptericin-LacZ/X; hs-GAL4/+. (K) diptericin-LacZ/X; hs-GAL4/EP(2)0578. (L) EP(X)1516/diptericin-LacZ; hs-GAL4/+. Columns: CTL, uninfected control samples; Microbe infected, E. coli-infected control samples; hs>DTRAF1, heat shock-induced DTRAF1-overexpressing samples; hs>DTRAF2, heat shock-induced DTRAF2-overexpressing samples.