TABLE 2.
Comparison of T-cell subsets in 43 pairsa of fresh and shipped samples analyzed at the JHU site
| Phenotype | Mean % ± SD in:
|
Statistical analysis (shipped − fresh)
|
|||
|---|---|---|---|---|---|
| Shipped samples | Fresh samples | Main effectb | 95% CIc | P valued | |
| CD4+ | 47.1 ± 10.8 | 47.0 ± 11.0 | −0.02 | −0.35, 0.31 | 0.91 |
| CD8+ | 18.7 ± 6.74 | 18.2 ± 7.68 | 0.11 | −0.30, 0.52 | 0.56 |
| CD26+ CD3+ | 46.2 ± 9.94 | 45.9 ± 10.3 | 0.23 | −0.64, 1.10 | 0.58 |
| CD26+ CD4+ | 34.2 ± 9.61 | 34.3 ± 9.74 | 0.09 | −0.54, 0.72 | 0.75 |
| CD26+ CD8+ | 12.0 ± 4.98 | 11.6 ± 6.10 | 0.14 | −0.20, 0.48 | 0.41 |
| CD25+ CD3+ | 13.8 ± 7.28 | 14.4 ± 7.06 | −0.71 | −1.39, −0.03 | 0.04 |
| CD25+ CD4+ | 10.3 ± 4.38 | 10.8 ± 4.61 | −0.68 | −1.23, −0.13 | 0.02 |
| CD25+ CD8+ | 3.49 ± 4.33 | 3.60 ± 4.66 | −0.03 | −0.24, 0.18 | 0.74 |
| DR+ CD4+ | 3.04 ± 1.36 | 3.03 ± 1.23 | 0.02 | −0.08, 0.12 | 0.71 |
| DR+ CD8+ | 4.61 ± 5.26 | 4.42 ± 5.96 | 0.08 | −0.12, 0.28 | 0.38 |
| CD38+ CD4+ | 25.0 ± 8.67 | 26.1 ± 9.18 | −0.47 | −0.83, −0.11 | 0.01 |
| CD38+ CD8+ | 6.04 ± 3.82 | 6.30 ± 4.14 | −0.13 | −0.31, 0.05 | 0.16 |
a
In 40 cases, there were two replicates per subject for both fresh and shipped samples.
b
The main-effect estimates for each parameter are not identical to the differences between the overall means for shipped samples and the overall means for fresh samples, because the overall means are based on all subjects while the statistical comparisons consider only the 40 subjects who had replicates for both fresh and shipped samples.
c
CI, confidence interval.
d
Based on the 40 subjects with two fresh replicates and two shipped replicates.