TABLE 3.

Intra-assay accuracy of GAPDH assay by real-time RT-PCR

Repetition no.	CT for amt of input RNA (ng)a
	1,000b	200c	40d	8e
1	16.20	18.22	20.64	22.73
2	16.11	18.13	20.77	22.54
3	15.96	18.34	20.96	22.54
4	16.17	18.25	20.75	22.68

^aInput RNA amount.

^bMean ± standard deviation, 16.11 ± 0.11; CV, 0.68%.

^cMean ± standard deviation, 18.24 ± 0.09; CV, 0.49%.

^dMean ± standard deviation, 20.78 ± 0.13; CV, 0.63%.

^eMean ± standard deviation, 22.62 ± 0.10; CV, 0.44%.