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. 2003 Nov;10(6):1051–1058. doi: 10.1128/CDLI.10.6.1051-1058.2003

FIG. 7.

FIG. 7.

Western blot analysis of recombinant proteins purified from clones 2D/16, B-55A/2, and 9B/30. (I) A, GST protein probed with anti-GST antibodies. B, fusion protein from clone 2D/16 probed with pooled sera from TB patients. C, fusion protein from clone 2D/16 probed with pooled sera from normal human subjects. (II) GST fusion protein from clone B55A/2 (lanes 1), recombinant protein digested with factor Xa (lanes 2), and expressed peptide after removal of GST tag (lanes 3) probed with pooled TB sera (A) or pooled normal human sera (B). (III) GST fusion protein from clone 9B/30 (lanes 1), recombinant protein digested with factor Xa (lanes 2), and expressed peptide after removal of GST tag (lanes 3) probed with pooled TB sera (A) or pooled normal human sera (B). Lanes C in panels II and III, controls. Sizes are indicated in kilodaltons. Arrows indicate the reactive bands.