TABLE 1.
Reagents used for measurement of cytokine levels with the Luminex Flowmetrix system
| Assay | Standard
|
Capture antibody
|
Detector antibody
|
|||||
|---|---|---|---|---|---|---|---|---|
| Cytokine | Source | Clone | Type | Bead set | Clone | Type | Concn (μg/ml) | |
| 4-Plex | IL-1β | R&D Systems | 2805.31 | Mouse IgG1a | 6947 | Polyclonal | Goat IgG | 20, 40 (cold)b |
| IL-8 | R&D Systems | 6217.111 | Mouse IgG1 | 6969 | Polyclonal | Goat IgG | 20, 80 (cold) | |
| IL-10 | R&D Systems | 23738.111 | Mouse IgG2b | 8047 | Polyclonal | Goat IgG | 120 | |
| IL-12 | R&D Systems | 24945.11 | Mouse IgG1 | 8053 | Polyclonal | Goat IgG | 80 | |
| 5-Plex | IL-2 | Pharmingen | 5344.111 | Mouse IgG1 | 6953 | B33-2 | Mouse IgG1 | 40 |
| IL-4 | Pharmingen | 8D4-8 | Mouse IgG1 | 6958 | MP4-25D2 | Rat IgG1 | 80 | |
| IL-6 | Pharmingen | MQZ-13A5 | Rat IgG1 | 6964 | MQZ-39C3 | Rat IgG2a | 120 | |
| IFN-γ | Pharmingen | NIB42 | Mouse IgG1 | 8058 | 4S.B3 | Mouse IgG1 | 120 | |
| TNF-α | Pharmingen | MAb1 | Mouse IgG1 | 8064 | MAb11 | Mouse IgG1 | 40 | |
a
IgG1, immunoglobulin G1.
b
The cold antibodies used came from the same source and clone but lacked the reporter dye.