FIG. 4.

Inhibition of PBMC proliferation of seven animals (six experimentally infected animals and one naturally exposed field reactor) to antigen (PPD-B and ESAT-6) and mitogen (ConA) by the VDR antibody. Anti-VDR and control antibodies were used at a final dilution of 1/400 in PBMC cultures. The results are expressed as the mean (plus standard deviation) inhibition of proliferation compared to cultures of PBMC plus antigen or mitogen (no antibody). The VDR antibody (solid bars) significantly inhibited the proliferative response of PBMC to both PPD-B and ESAT-6 compared with either control antibody (IgG2b [shaded bars] or IgG [open bars]) (P < 0.0001 in all cases).