FIG. 7.

IFN-γ and GM-CSF mRNA levels in SEC-stimulated and nonstimulated PBMC. PBMC were cultured with or without SEC (1 μg/ml) for 4 h. IFN-γ and GM-CSF mRNA levels were measured by the RT-PCR method. (A) Representative amplification of RT-PCR from freshly isolated (top), nonstimulated (middle), and SEC-stimulated (bottom) PBMC. RT-PCR products were detected by electrophoresis with 3.0% agarose followed by staining with ethidium bromide. Lane M, molecular weight marker; lane 1, IFN-γ (270 bp); lane 2, GM-CSF (429 bp); lane 3, β-actin (405 bp). (B) Relative units after normalization to the β-actin mRNA level. The results shown are means ± SD from three separate experiments. Values with different letters are significantly different (P < 0.05).