Figure 2.

Effect of overexpression of Jun and Fos proteins on TRE-, hARE-, and mutant hARE-mediated CAT gene expression. (A) The Hep-G2 cells were cotransfected with 10 μg of reporter plasmids TRE–tk–CAT (Left), hARE–tk–CAT (Center), and mutant hARE–tk–CAT (Right) with 10 μg of expression plasmids LNCX, LNCX–Jun and LNCX–c-Fos individually and in combinations as shown in separate experiments. The LNCX–c-Fos was also used in different concentrations (μg). (B) HeLa and F9 cells were cotransfected with 10 μg of reporter plasmid hARE–tk–CAT and different concentration of c-Fos expression plasmid LNCX–c-Fos. Five micrograms of RSV–β-gal plasmid was included in each case as internal control of transfection efficiency. Forty-eight hours after transfection, the cells were analyzed for β-gal and CAT activities.