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Detection of TNF-α or IL-6 by ELISA in extracting buffers containing various nonionic detergents. Solutions of TNF-α (36 or 107 pg/ml) or IL-6 (100 or 300 pg/ml) were diluted 1:10 with kit diluent before assaying. Igepal (1%) and Triton X-100 (0.25%) inhibited the detection of TNF-α, whereas IL-6 detection was similar in all buffers tested. The data are means ± SEM and are expressed as percent absorbance values obtained with PBS (diluted 1:10) in kit diluent.
