FIG. 3.

Efficiency of IL-6 removal from the insoluble fraction of synovial tissue when extracted with 0.1% Igepal. Western blotting was performed on whole tissue or the insoluble pellet remaining after 0.1% Igepal extraction and developed with anti-actin or anti-human IL-6, and protein amounts were quantified by densitometry. For comparison, the total protein (Tot Prot) amounts in tissue and pellet lysate are shown. Less than 20% of IL-6 remained in the insoluble fraction after the extraction procedure. The data are means ± SEM of the results from two RA synovia and are expressed as percentages of the amounts in whole tissue.