Figure 1.

Structure of recombinant Lhcb transgene. A cassette containing transcriptional regulatory elements (open boxes) and a recombinant Lhcb gene that carries an affinity tag of six histidines (asterisk) was inserted into the binary vector pBIN19. To direct the precursor protein into plastids, the endogenous Lhcb transit sequence was replaced by the pea rbcS transit sequence (solid box). In frame the Lhcb gene was constructed from synthetic oligonucleotides (horizontal stripes) and from the C-terminal half of a pea Lhcb gene (vertical stripes).