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Southern analysis of the myb1 gene with genomic DNAs from several Nicotiana species. The genomic DNAs (15 μg) of the amphidiploid N. tabacum (cvs. Xanthi nc and Xanthi) and its parental diploid species, N. sylvestris and N. tomentosiformis were digested with EcoRI (lanes 1, 3, 5, and 7) or HindIII (lanes 2, 4, 6, and 8). After blotting, the DNAs were hybridized with a gene-specific myb1 probe.
