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. 2003 Nov;41(11):5134–5146. doi: 10.1128/JCM.41.11.5134-5146.2003

TABLE 2.

LC-PCR primers and the LC-PCR sensitivities

Species and subgroups Target gene (description) PCR primer and sequence (5′-3′) Product size (bp) Tma Sensitivityb
Reference
Direct LC-PCR LC-PCR after enrichment
Escherichia coli
    EPEC and EHEC eaeA (E. coli attaching and effacing) EAE-a, ATGCTTAGTGCTGGTTTAGG 248 78.2 ± 0.2 3 3.3 37
EAE-b, GCCTTCATCATTTCGCTTTC
    EHEC stx1 (Shiga toxin) JMS1F, GTCACAGTAACAAACCGTAACA 95 81.4 ± 1.0 4.1 2.6 14
JMS1R, TCGTTGACTACTTCTTATCTGGA
stx2 JMS2F, CGACCCCTCTTGAACATA 108 81.4 ± 0.3 4 2 14
JMS2R, GATAGACATCAAGCCCTCGT
    ETEC (LT) LT (heat-labile enterotoxin) LT-1, AGCAGGTTTCCCACCGGATCACCA 132 81.4 ± 0.3 4.3 2.3 12
LT-2, GTGCTCAGATTCTGGGTCTC
    ETEC (ST) ST (heat-stable enterotoxin) STa-F, GCTAATGTTGGCAATTTTTATTTCTGTA 190 76.6 ± 0.2 4.5 3.5 6
STa-R, AGGATTACAACAAAGTTCACAGCAGTAA
    EAEC astA (EAECheat-stable enterotoxin) EAST-1S, GCCATCAACACAGTATATCC 106 84.9 ± 0.6 3.6 2 40
EAST-1AS, GAGTGACGGCTTTGTAGTCC
aggR (transcriptional activator for EAEC aggregative adherence fimbria I expression) aggRks1, GTATACACAAAAGAAGGAAGC 254 78.9 ± 0.2 4.6 2 27
aggRkas2, ACAGAATCGTCAGCATCAGC
    EIEC virA (virulence of plasmid) virA-F, CTGCATTCTGGCAATCTCTTCACA 215 81.4 ± 0.3 3.3 6 34
virA-R, TGATGAGCTAACTTCGTAAGCCCTCC
Shigella spp. virA 4.8 6
Salmonella spp. invA (invasion) invA 139, GTGAAATTATCGCCACGTTCGGGCAA 284 85.8 ± 0.4 5 1.3 26
invA 141, TCATCGCACCGTCAAAGGAACC
Yersinia enterocolitica yadA (Yersinia adhesion) yadA667-F, TGTTCTCATCTCCATATGC 203 85.5 ± 0.8 4.5 2.9 This study
yadA851-R2, TCCTTTCGCTGCTTCAGCA
Y. pseudotuberculosis yadA >203 84.3 ± 0.3 5.7 1.6
Campylobacter jejuni gyrA (DNA gyrase: a primary target of the fluoroquinolone antibiotics) JL238, TGGGTGCTGTTATAGGTCGT 192 80.3 ± 0.3 3 2 39
JL239, GCTCATGAGAAAGTTTACTC
Vibrio cholerae RTX toxin (repeat in toxin) rtxC-F, CGACGAAGATCATTGACGAC 265 85.8 ± 0.5 5 2 3
rtxC-R, CATCGTCGTTATGTGGTTGC
CT (cholera toxin) CT-F, ACAGAGTGAGTACTTTGACC 308 80.4 ± 0.3 4.8 2.3 22
CT-R, ATACCATCCATATATTTGGGAG
V. parahaemolyticus tdh (thermostable direct hemolysin) Tdh199-F, GGTACTAAATGGCTGACATC 251 80.4 ± 0.4 4.7 2 24
Tdh199-R, CCACTACCACTCTCATATGC
trh (tdh-related hemolysin) Trh250-F, GGCTCAAAATGGTTAAGCG 250 79.6 ± 0.1 4.7 2 24
Trh251-R, CATTTCCGCTCTCATATGC
V. vulnificus Cytotoxin hemolysin VV-1, CTCACTGGGGCAGTGGCT 383 84.4 ± 0.3 5 1.9 38
VV-2, CCAGCCGTTAACCGAACCA
Aeromonas hydrophila Cytolytic enterotoxin AHCF1, GAGAAGGTGACCACCAAGAACA 232 89.0 ± 0.5 4.7 4 16
AHCR1, AACTGACATCGGCCTTGAACTC
Clostridium perfringens Enterotoxin GAP11, GGTTCATTAATTGAAACTGGTG 154 76.0 ± 0.6 3.9 3.8 15
GAP12, AACGCCAATCATATAAATTACAGC
Staphylococcus aureus Nuclease SA-1, GCGATTGATGGTGATACGGTT 276 79.0 ± 0.3 4.5 6.9 2
SA-2, CAAGCCTTGACGAACTAAAGC
Bacillus cereus Hemolysin BC-1, CTGTAGCGAATCGTACGTATC 185 79.8 ± 0.9 3.9 8 38
BC-2, TACTGCTCCAGCCACATTAC
a

Values represent means ± standard deviations of 10 to 18 tests.

b

Sensitivity values represent the minimum numbers of cells necessary for the assay to yield positive LC-PCR results. Values are given as number of cells log10 per gram.