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Analyses of A. pullorum-specific PCR. Lanes 1 through 3, A. pullorum Texas DNA from the 20-year old slides, Anaplasma marginale Florida strain DNA, and Anaplasma phagocytophilum HZ strain DNA, respectively; lanes 4 and 5, first-step PCR and the nested-PCR template-less negative controls; lane M, 1-kb Plus DNA ladder.
