Figure 3.

Southern blot hybridization analysis of DNA from kanamycin-resistant calli derived from cells bombarded with control plasmids. pNeoRBLuc plasmid (lanes A–F) and the borderless construct pNeoLuc together with p35SD1 and p35SD2 plasmids (lanes G and H) were delivered to maize cells. The blot was hybridized with the neo probe. NT, DNA from nontransformed tobacco cells. Lanes 1, 5, and 10: genomic DNA mixed with pNeoRBLuc to reconstruct the integration of 1, 5, or 10 copies of the gene per diploid nucleus, respectively.