Table 1.
Activity of virD1 and virD2 in tobacco suspension cells
| Plasmids + pGUS | Mean ± SD | % of control |
|---|---|---|
| pRB(+)Luc | 1.36 ± 0.06 | — |
| pRB(+)Luc + p35SD1 | 0.98 ± 0.03 | 72 |
| pRB(+)Luc + p35SD2 | 0.69 ± 0.07 | 50 |
| pRB(+)Luc + p35SD1 + p35SD2 (1:1:1) | 0.27 ± 0.05 | 20 |
| pRB(+)Luc + p35SD1 + p35SD2 (1:2:2) | 0.14 ± 0.05 | 10 |
| pRB(+)Luc + p35SD1 + p35SD2(rev) (1:1:1) | 1.08 ± 0.13 | 80 |
| pRB(+)Luc + p35SD1 + p35SD2(rev) (1:2:2) | 1.13 ± 0.08 | 83 |
| pRB(−)Luc | 1.40 ± 0.14 | — |
| pRB(−)Luc + p35SD1 | 1.33 ± 0.13 | 95 |
| pRB(−)Luc + p35SD2 | 1.19 ± 0.12 | 85 |
| pRB(−)Luc + p35SD1 + p35SD2 | 1.56 ± 0.38 | 112 |
Plasmid constructs are described in the legend to Fig. 1 and were delivered to tobacco cells by the biolistic device. Numbers in parentheses indicate the molar ratio of plasmids. Following incubation for 24 hr, tissues were homogenized and enzyme activities determined. Activities are expressed as a ratio of luciferase (Luc) to β-glucuronidase (GUS). Independent bombardments were analyzed, and data are presented as mean values of six repetitions ± SD. % control values are determined from the ratio of the luciferase to GUS activities to those activities observed with control plasmid.