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. 2003 Nov;41(11):4971–4979. doi: 10.1128/JCM.41.11.4971-4979.2003

FIG. 2.

FIG. 2.

Two ELMA detection strategies. (a) Type A. The 3′ end of the detection primer is designed to reach exactly the detection point. (Left) The corresponding biotinylated dNTP is incorporated at the 3′ end of the probe if the 3′ end of the probe matches with the target DNA. (Right) There will be no incorporation in the case of mismatch. Thus, two probes, a wild-type-specific and a mutant-specific probe, are used for the assay. (b) Type B. The 3′ end of the detection primer is designed to reach 1 base before the detection point. The mutation pattern is determined by the type of biotinylated dNTP (dATP or dTTP) incorporated at the detection point. Therefore, the probe in the type B strategy is not type specific.