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. 2003 Nov;41(11):4971–4979. doi: 10.1128/JCM.41.11.4971-4979.2003

TABLE 2.

Primers used for amplification of target DNA and probes for hybridization

Enzyme targeted Position ELMA type Fragment Primer for amplification
Probe for hybridization
Name Directiona Sequence Pattern Name Sequence
Protease 30 A a PRO1F Senses ATA GAC AAG GAA CTG TAT CC D30 PRO30P-W Amine-AGG AAG CTC TAT TAG ATA CAG GAG CAG ATG
PRO1R2 Antisense AAA TTC ATT TCT TCT AAT ACT GT N30 PRO30P-M Amine-AGG AAG CTC TAT TAG ATA CAG GAG CAG ATA
46 A b PRO2F Sense GCC AGG AAG ATG GAA ACC AA M46 PRO46P-WC Amine-TTT GAT AAA ACC TCC AAT TCC CMC TAC CAT
PRO2R Antisense TGT AGG TCC TAC TAA TAC TG M46 PRO46P-WA Amine-TTT GAT AAA ACC TCC AAT TCC CMC TAA CAT
146 PRO46P-MTTG Amine-TTT GAT AAA ACC TCC AAT TCC CMC TAT CAA
146 PRO46P-MATA Amine-TAC TTT GAT AAA ACC TCC AAT TCC CMC TAT
48 A G48 PRO48P-W Amine-TCT TAC TTT GAT AAA ACC TCC AAT TCC CCC
V48 PRO48P-M Amine-TCT TAC TTT GAT AAA ACC TCC AAT TCC CAC
82 A c PRO3F Sense ATA CCC ATA GAA ATC TGT GG V82 PRO8P-2W Amine-GGT ACA GTA TTA GTA GGA CCT ACA CCT GTC
PRO3R Antisense GGA AAA TTT AAA GTG CAA CCA A A82 PRO82P-M Amine-GGT ACA GTA TTA GTA GGA CCT ACA CCT GCC
84 A I84 PRO84P-W Amine-CAG TAT TAG TAG GAC CTA CAC CTG TCA AYA
V84 PRO84P-M Amine-CAG TAT TAG TAG GAC CTA CAC CTG TCA AYG
90 A L90 PRO90P-W Amine-CAC CTG TCA ACA TAA TTG GAA GAA ATC TGT
M90 PRO90P-M Amine-CAC CTG TCA ACA TAA TTG GAA GAA ATC TGA
RT 41 A d RT1/2F Sense GTT AAA CAA TGG CCA TTG ACA GA M41 RT41P-W Amine-TAA AAG CAT TAG TAG AAA TTT GTA CAG AAA
RT1R Antisense GTA TGG ATT TTC AGG CCC AAT T L41 RT41P-M Amine-TAA AAG CAT TAG TAG AAA TTT GTA CAG AAC
L41 RT41P-MT Amine-TAA AAG CAT TAG TAG AAA TTT GTA CAG AAT
67 A e RT1/2F Sense GTT AAA CAA TGG CCA TTG ACA GA D67 RT67P-W Amine-ATA CTC CAG TGT TTG CCA TAA AGA AAA ARG
RT2R Antisense TGA ACT TCC CAG AAG TCT TGA G N67 RT67P-M Amine-ATA CTC CAG TGT TTG CCA TAA AGA AAA ARA
70 A K70 RT70P-W Amine-GTT CTC TGA AAT CTA CTA ATT TTC TCC ATT
R70 RT70P-M Amine-GTT CTC TGA AAT CTA CTA ATT TTC TCC ATC
184 A f RT3F Sense AGC ATG ACA AAA ATC TTA GAG CC M184 RT1840P-W Amine-AAA ATC CAG ACA TAG TTA TCT ATC AAT ACA
RT3R Antisense TAT TTC TAA GTC AGA TCC TAC ATA V184 RT184P-M Amine-AAA ATC CAG ACA TAG TTA TCT ATC AAT ACG
215 B g RT4F Sense GCA GCA TAG AAC AAA AAT AGA GG T215Y RT215-P Amine-CTG AGA CAA CAT CTG TTG AGG TGG GGA TTT
RT4R Antisense TAT CAG GAT GGA GTT CAT AAC C