TABLE 3.
Detection end points of wild-type ELMA probes
| Codon | Target | ODa of probe-target pair at the following copy number of the target:
|
Meanc | SDd | Cutoffb (mean + 3 SD) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| 106 | 105 | 104 | 103 | 102 | 101 | |||||
| 41 | Wild type | >2.000 | >2.000 | >2.000 | >2.000 | >2.000 | 1.942 ± 0.117 | 0.212 | ||
| Mutant | 0.103 ± 0.010 | 0.103 ± 0.073 | 0.121 ± 0.063 | 0.096 ± 0.005 | 0.096 ± 0.009 | 0.105 ± 0.007 | 0.104 | 0.036 | ||
| 67 | Wild type | >2.000 | >2.000 | >2.000 | >2.000 | >2.000 | 1.952 ± 0.096 | 0.311 | ||
| Mutant | 0.193 ± 0.027 | 0.210 ± 0.016 | 0.225 ± 0.022 | 0.249 ± 0.009 | 0.233 ± 0.015 | 0.165 ± 0.024 | 0.212 | 0.033 | ||
| 70 | Wild type | 1.553 ± 0.344 | 1.673 ± 0.385 | 1.587 ± 0.349 | 1.828 ± 0.344 | 1.903 ± 0.195 | 1.736 ± 0.260 | 0.125 | ||
| Mutant | 0.093 ± 0.007 | 0.059 ± 0.009 | 0.075 ± 0.007 | 0.056 ± 0.001 | 0.070 ± 0.012 | 0.094 ± 0.010 | 0.074 | 0.017 | ||
| 184 | Wild type | >2.000 | >2.000 | >2.000 | >2.000 | >2.000 | 1.840 ± 0.321 | 0.560 | ||
| Mutant | 0.088 ± 0.008 | 0.078 ± 0.002 | 0.088 ± 0.007 | 0.091 ± 0.027 | 0.061 ± 0.007 | 0.256 ± 0.369 | 0.110 | 0.150 | ||
| 215 | Wild type | >2.000 | >2.000 | 1.949 ± 0.067 | 0.585 ± 0.097 | 0.085 ± 0.041 | 0.041 ± 0.006 | 0.171 | ||
| Mutant | 0.041 ± 0.007 | 0.078 ± 0.067 | 0.082 ± 0.065 | 0.044 ± 0.004 | 0.029 ± 0.007 | 0.034 ± 0.005 | 0.051 | 0.040 | ||
| 30 | Wild type | >2.000 | >2.000 | >2.000 | >2.000 | 1.810 ± 0.275 | 0.410 ± 0.131 | 0.521 | ||
| Mutant | 0.305 ± 0.031 | 0.269 ± 0.027 | 0.273 ± 0.025 | 0.310 ± 0.033 | 0.150 ± 0.029 | 0.056 ± 0.017 | 0.227 | 0.098 | ||
| 46 | Wild type | >2.000 | >2.000 | >2.000 | >2.000 | >2.000 | 1.464 ± 0.681 | 0.156 | ||
| Mutant | 0.084 ± 0.011 | 0.094 ± 0.008 | 0.096 ± 0.007 | 0.103 ± 0.018 | 0.076 ± 0.011 | 0.034 ± 0.009 | 0.081 | 0.025 | ||
| 48 | Wild type | >2.000 | >2.000 | >2.000 | >2.000 | >2.000 | 1.464 ± 0.659 | 1.455 | ||
| Mutant | 0.448 ± 0.014 | 0.411 ± 0.041 | 0.415 ± 0.041 | 0.435 ± 0.057 | 0.735 ± 0.170 | 1.123 ± 0.216 | 0.594 | 0.287 | ||
| 82 | Wild type | >2.000 | >2.000 | 1.682 ± 0.637 | 1.937 ± 0.120 | 0.379 ± 0.376 | 0.602 ± 0.981 | 0.328 | ||
| Mutant | 0.184 ± 0.034 | 0.164 ± 0.013 | 0.166 ± 0.014 | 0.171 ± 0.029 | 0.041 ± 0.005 | 0.036 ± 0.007 | 0.127 | 0.067 | ||
| 84 | Wild type | >2.000 | >2.000 | 1.756 ± 0.489 | >2.000 | 0.611 ± 0.509 | 0.677 ± 0.957 | 0.284 | ||
| Mutant | 0.141 ± 0.019 | 0.134 ± 0.014 | 0.135 ± 0.013 | 0.162 ± 0.004 | 0.229 ± 0.018 | 0.201 ± 0.012 | 0.167 | 0.039 | ||
| 90 | Wild type | >2.000 | >2.000 | >2.000 | 1.936 ± 0.129 | 1.261 ± 0.863 | 0.082 ± 0.055 | 0.213 | ||
| Mutant | 0.134 ± 0.013 | 0.126 ± 0.009 | 0.125 ± 0.011 | 0.086 ± 0.010 | 0.039 ± 0.006 | 0.048 ± 0.005 | 0.093 | 0.040 | ||
a
Average ± SD based on quadruplicated data.
b
Determined from OD of mismatched probe-target pairs (wild-type probe and mutant target). The lowest copy number with an OD higher than the cutoff is designated the end point of the detection (indicated by boldfaced OD).
c
Mean OD of mismatched probe-target pairs.
d
SD of mismatched probe-target pairs.