TABLE 1.
Anti-Toxoplasma antibody titers and results of conventional and real-time T. gondii PCR on sequential samples of blood and CSF
| Sample collection daya | Anti-Toxoplasma serology
|
No. of tachyzoites/ml inc:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IgG titer (IU/ml) | IgM (index)b | Whole blood
|
Buffy coat
|
Serum
|
CSF
|
|||||
| PCR-ELISA | Real-time PCR | PCR-ELISA | Real-time PCR | PCR-ELISA | Real-time PCR | PCR-ELISA | Real-time PCR | |||
| −127 | 2,400 | 0 | 0 | 0 | ||||||
| −15 | 2,400 | 0 | 0 | 0 | ||||||
| −8 | 0 | 0 | ||||||||
| −1 | 0 | 0 | ||||||||
| +7 | 0 | 0 | ||||||||
| +14 | 0 | 0 | ||||||||
| +20 | 0 | 0 | ||||||||
| +27 | 0 | 0 | ||||||||
| +34 | 0 | 0 | ||||||||
| +36 | 0 | 0 | + | 86 | ||||||
| +41 | + | 26 | ||||||||
| +43 | + | 2,200 | + | 26,000 | + | 37 | ||||
| +48 | + | 530 | + | 2,200 | + | 19 | ||||
| +50 | + | 130 | + | 1,400 | ||||||
| +53 | + | 240 | + | 1,400 | ||||||
| +55 | + | 0 | ||||||||
| +61 | 0 | 0 | + | 11 | ||||||
| +62 | + | 18 | ||||||||
| +67 | 300 | 0 | + | 0 | + | 8 | + | 0 | ||
| +69 | + | 16 | 0 | 0 | ||||||
| +71 | + | 0 | + | 6 | ||||||
| +74 | 0 | 19 | 0 | 0 | 0 | 0 | ||||
| +81 | 160 | 0 | 0 | 0 | + | 0.5 | + | 0 | ||
| +84 | 0 | 0 | 0 | 0 | ||||||
| +91 | 0 | 37 | + | 0 | 0 | 0 | ||||
| +99 | 0 | 0 | ||||||||
| +102 | 250 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| +106 | 0 | 0 | 0 | 0 | ||||||
| +126 | 0 | 0 | ||||||||
| +165 | 240 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| +179 | 198 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| +197 | 0 | 0 | 0 | 0 | ||||||
| +200 | 187 | 0 | 0 | 0 | ||||||
| +214 | 0 | 0 | 0 | 0 | ||||||
| +225 | 0 | 0 | 0 | 0 | ||||||
− and + indicate days before and after SCT, respectively.
Values were calculated according to the manufacturer’s instructions.
+, PCR-ELISA positive.