TABLE 1.
Comparison of yeast identifications made using the API 20C AUX method and the MicroSeq D2 LSU rDNA sequencing kit
| Phenotypic identification | Sequence-based identification using D2 database | No. of isolates | No. of isolates with concordant results | Distance score range (%) |
|---|---|---|---|---|
| Candida albicans | Candida albicans | 12 | 12 | 0.0-0.92 |
| Candida ciferrii | Stephanoascus ciferriia | 3 | 3 | 0-0.31 |
| Candida colliculosa | Torulaspora delbrueckiia | 1 | 1 | 0 |
| Candida dubliniensisb | Candida albicans | 1 | 0 | 3.42 |
| Candida famata | Debaryomyces hanseniia | 1 | 1 | 0 |
| Candida glabrata | Candida glabrata | 11 | 11 | 0.30-0.90 |
| Candida guilliermondii | Yamadazyma guilliermondii/Candida xestobiia,c | 5 | 5 | 0-1.17 |
| Candida kefyr | Kluyveromyces marxianus/lactisa,c | 1 | 1 | 0 |
| Candida krusei | Issatchenkia orientalisa | 8 | 8 | 0 |
| Candida lipolytica | Yarrowia lipolyticaa | 8 | 8 | 0-0.36 |
| Candida lusitaniae | Candida lusitaniae | 10 | 10 | 0-1.06 |
| Candida norvegensis | Candida norvegensis | 1 | 1 | 0 |
| Candida parapsilosis | Candida parapsilosis/Candida osornesisc | 14 | 14 | 0-1.88 |
| Candida pelliculosa | Pichia anomala-1a | 2 | 1 | 0 |
| Pichia fabianii-1 | ||||
| Candida rugosa | Candida rugosa | 2 | 2 | 0.40 |
| Candida tropicalis | Candida tropicalis | 13 | 13 | 0-0.31 |
| Candida utilis | Pichia jadinii | 1 | 1 | 1.07 |
| Candida viswanathii | Candida lodderaea | 3 | 3 | 0.47 |
| Candida zeylanoides | Candida zeylanoides | 2 | 2 | 0 |
| Cryptococcus laurentii | Cryptococcus laurentii | 2 | 2 | 0-3.48 |
| Cryptococcus neoformans | Filobasidiella neoformansa | 7 | 7 | 0-0.86 |
| Cryptococcus uniguttulatus | Filobasidium uniguttulatuma | 1 | 1 | 0 |
| Debaryomyces hansenii | Debaryomyces hansenii | 1 | 1 | 0 |
| Kloeckera apis/Kloeckera apiculatad | Kloeckera apiculata | 1 | 1 | 0 |
| Malassezia pachydermatis | Malassezia pachydermatis | 1 | 1 | 0 |
| Pichia ohmeri | Pichia ohmeri | 2 | 2 | 0 |
| Rhodotorula glutinisb | Sporidiobolus johnsonii | 1 | 0 | 8.72 |
| Rhodotorula mucilaginosa | Rhodotorula mucilaginosa | 1 | 1 | 0.31 |
| Saccharomyces cerevisiae | Zygosaccharomyces fermentati | 12 | 10 | 0-0.31 |
| Debaryomyces hansenii | ||||
| Trichosporon mucoidesb | Trichosporon jirovecii | 3 | 0 | 0.0-3.78 |
a
Teleomorph.
b
Not in D2 database.
c
Indistinguishable by D2.
d
Indistinguishable by 20C.