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. 2003 Nov;41(11):5085–5093. doi: 10.1128/JCM.41.11.5085-5093.2003

FIG. 1.

FIG. 1.

Examples of electrophoretic profiles, as observed in 2% (A) or 1.4% (B) agarose gels of the Adiavet App, dsbE-like (8), omlA (34), cps-cpx (28), and aroA (21) PCR tests. Reactions were carried out with no DNA (lanes 1, 7, 13, 19, and 25) or 1 μg of purified genomic DNA from A. pleuropneumoniae serotype 5 (lanes 2, 8, 14, 20, and 26), A. pleuropneumoniae serotype 7 (lanes 3, 9, 15, 21, and 27), A. lignieresii (lanes 4, 10, 16, 22, and 28), A. suis ATCC 15557 (lanes 5, 11, 17, 23, and 29), and “A. porcitonsillarum” (lanes 6, 12, 18, 24, and 30). Lanes M, 100-bp ladder (Invitrogen); arrowheads indicate the position of the 600-bp band of this ladder; lane MA, molecular weight marker provided with the Adiavet App PCR test. Bands of this size marker allow rapid assignment of the amplicons generated to one of the four Adiavet App PCR typing groups. The amplicons observed in lanes 1, 5, and 6 correspond to the internal inhibition control of the Adiavet App PCR test.