TABLE 1.
Sensitivities of the different PCR tests and IMS isolation for detection of A. pleuropneumoniae from experimentally infected tonsils
| PCR test (reference) | Sensitivity (CFU/g of tonsil)a
|
|
|---|---|---|
| Direct PCRb | After-culture PCRc | |
| apxIVA (35) | 3.6 × 102 | 2.2 × 102 |
| Adiavet Appd (8) | 1.7 × 104 | 2.2 × 102 |
| omlA (34) | 4 × 104 | 2.2 × 102 |
| dsbE-like (8) | 4.8 × 105 | 5.2 × 102 |
| aroA (21) | <109 | 2.2 × 105 |
| Multiplex omlA (18) | 3.4 × 106 | 2.2 × 102 |
| cps-cpx (28) | 1.8 × 107 | 5.2 × 102 |
| Sirois et al. (37) | 2.1 × 107 | 2.2 × 102 |
a
A. pleuropneumoniae serotype 1 reference strain Shope 4074 was used to infect the tonsils. The sensitivity of IMS isolation was 4.39 × 103 CFU/g of tonsil.
b
PCR was carried out with DNA obtained without culture of the sample.
c
PCR was carried out with DNA obtained after culture of the sample.
d
Commercial PCR test.