TABLE 2.

Effects of time and storage conditions on Adiavet App PCR detection of A. pleuropneumoniae from whole tonsils of experimentally infected animals

Storage temp (°C)	Method	No. of animals positive after storage for the following times (days)a:
		2	4	8	17	120
RTb	Direct PCRc	4	4	3d	ND	NDe
	After-culture PCRf	0	1	0d	ND	ND
4°	Direct PCR	4	4	4	4	ND
	After-culture PCR	4	4	4	4	ND
−20	Direct PCR	ND	ND	4	4	4
	After-culture PCR	ND	ND	4	4	4

^aA total of four animals were tested.

^bRT, room temperature.

^cPCR was carried out with DNA obtained without culture of the sample.

^dOnly three animals were tested.

^eND, not done.

^fPCR was carried out with DNA obtained after culture of the sample.