TABLE 3.

Results of standard isolation, IMS isolation, and PCR detection (omlA and Adiavet App PCR tests) of A. pleuropneumoniae with whole tonsils or tonsil biopsy specimens from animals originating from infected herds

Group and specimen	No. of animals positive
	Isolation		PCR
			Directb		After culturec
	Standard	IMSa	omlAd	Adiavet App	omlA	Adiavet App
Group 3 (n = 36)
Whole tonsils	8	23	22e	34	35e	35e
Biopsies	NDf	ND	12e	28	15e	22e
Total positiveg	8	23	25	35	35	35
Group 4 (n = 39)
Whole tonsils	3	5	8	12	20e	12
Biopsies	ND	ND	3	9	6e	10
Total positive	3	5	8	14	21	14
Group 5 (n = 39)
Whole tonsils	0	4	0	3	6	7
Biopsies	ND	ND	0	0	2	4
Total positive	0	4	0	3	7	9
All positive groups (n = 114)
Whole tonsils	11	32	30e	49	61e	54e
Biopsies	ND	ND	15e	37	23e	36e
Total positive	11	32	33	52	63	58

^aIMS for serotype 5 (groups 3 and 4) or serotype 7 (group 5).

^bPCR was carried out with DNA obtained without culture of the sample.

^cPCR was carried out with DNA obtained after culture of the sample.

^dAs described by Savoye et al. (34).

^eSignificant differences in detection between whole tonsils and tonsils biopsy specimens (P < 0.05).

^fND, not done.

^gNumber of positive animals for each test, regardless of the sample used.