TABLE 4.

Sensitivities, specificities, and agreement (κ) of standard isolation technique, IMS isolation, and PCR tests (omlA and Adiavet App PCRs) for detection of A. pleuropneumoniae from whole tonsils

Test	% Positive samples	Results of comparison with the following benchmark tests:
		All testsa			Isolation						PCR
											Directb						After culturec
					Standard			IMS			omlAd			Adiavet App			omlA			Adiavet App
		SEe (%)	SPf (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ
Standard isolation	10	17	100	0.15				31	99	0.38	37	100	0.46	22	100	0.25	18	100	0.17	20	100	0.21
IMS isolation	28	49	100	0.46	91	79	0.38				83	91	0.73	61	97	0.61	49	96	0.44	56	97	0.53
omlA PCR, direct	26	46	100	0.42	100	82	0.46	78	94	0.73				61	100	0.64	49	100	0.47	55	100	0.57
Adiavet App PCR, direct	43	75	100	0.73	100	63	0.25	94	77	0.61	100	77	0.64				77	96	0.72	87	97	0.84
omlA PCR, after culture	53	93	100	0.93	100	51	0.17	94	62	0.44	100	63	0.47	96	78	0.72				98	86	0.84
Adiavet App PCR, after culture	47	83	100	0.81	100	58	0.21	94	71	0.53	100	71	0.57	96	89	0.84	87	98	0.84

^aA sample was considered positive if A. pleuropneumoniae was detected by at least one assay; a sample was considered negative if A. pleuropneumoniae was not detected by any of the assays.

^bPCR was carried out with DNA obtained without culture of the sample.

^cPCR was carried out with DNA obtained after culture of the sample.

^dAs described by Savoye et al. (34).

^eSE, sensitivity. The sensitivity of a test was defined as the number of samples positive by the test that were also positive by the benchmark test divided by the total number of samples positive by the benchmark test.

^fSP, specificity. The specificity of a test was defined as the number of samples negative by the test that were also negative by the benchmark test divided by the total number of samples negative by the benchmark test.