TABLE 5.

Sensitivities, specificities, and agreement (κ) of standard isolation technique, IMS isolation, and PCR tests (omlA and Adiavet App PCRs) for detection of A. pleuropneumoniae from tonsil biopsy specimens^a

Test	% Positive samples	Results of comparison with the following benchmark tests:
		All tests			PCR
					Directb						After culturec
					omlAd			Adiavet App			omlA			Adiavet App
		SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ	SE (%)	SP (%)	κ
omlA PCR, direct	13	31	100	0.34				37	97	0.41	43	93	0.41	29	93	0.26
Adiavet App PCR, direct	32	75	100	0.78	87	77	0.41				77	78	0.44	74	87	0.61
omlA PCR, after culture	20	47	100	0.49	60	87	0.41	46	93	0.44				58	99	0.64
Adiavet App PCR, after culture	31	73	100	0.76	67	74	0.26	72	88	0.61	95	83	0.64

^aSee footnotes a, e, and f of Table 4 for definitions of all tests, SE, and SP, respectively.

^bPCR was carried out with DNA obtained without culture of the sample.

^cPCR was carried out with DNA obtained after culture of the sample.

^dAs described by Savoye et al. (34).