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. 2003 Nov;41(11):5085–5093. doi: 10.1128/JCM.41.11.5085-5093.2003

TABLE 5.

Sensitivities, specificities, and agreement (κ) of standard isolation technique, IMS isolation, and PCR tests (omlA and Adiavet App PCRs) for detection of A. pleuropneumoniae from tonsil biopsy specimensa

Test % Positive samples Results of comparison with the following benchmark tests:
All tests
PCR
Directb
After culturec
omlAd
Adiavet App
omlA
Adiavet App
SE (%) SP (%) κ SE (%) SP (%) κ SE (%) SP (%) κ SE (%) SP (%) κ SE (%) SP (%) κ
omlA PCR, direct 13 31 100 0.34 37 97 0.41 43 93 0.41 29 93 0.26
Adiavet App PCR, direct 32 75 100 0.78 87 77 0.41 77 78 0.44 74 87 0.61
omlA PCR, after culture 20 47 100 0.49 60 87 0.41 46 93 0.44 58 99 0.64
Adiavet App PCR, after culture 31 73 100 0.76 67 74 0.26 72 88 0.61 95 83 0.64
a

See footnotes a, e, and f of Table 4 for definitions of all tests, SE, and SP, respectively.

b

PCR was carried out with DNA obtained without culture of the sample.

c

PCR was carried out with DNA obtained after culture of the sample.

d

As described by Savoye et al. (34).