TABLE 2.
Characterization of binary toxin-positive strainsa
| REA type | Isolate designation | LCT production
|
PCR
|
Toxinotyping
|
Patient data | ||||
|---|---|---|---|---|---|---|---|---|---|
| TcdA | TcdB | cdt genes | PaLocb | B1 HincII/AccIc | A3 EcoRIc | Toxinotype | |||
| AA2 | 4380 | − | + | + | + | 3 | 8 | V | Asymptomatic patient |
| AA1 | 5943 | − | − | + | + | − | 5 | XIa | No data |
| AA1 | 4680 | − | − | + | + | − | 5 | XIa | Symptomatic patient |
| AA1 | 3126 | − | − | + | + | − | 8 | XIb | Asymptomatic patientd |
| AA1 | 3133 | − | − | + | + | − | 8 | XIb | Asymptomatic patientd |
| AA1 | 3800 | − | − | + | + | − | 8 | XIb | Asymptomatic patient |
| AM1 | 3859 | − | − | + | − | − | − | Tox− | Hospital environment |
| CQ1 | 3376 | − | − | + | − | − | − | Tox− | Hospital environment |
| CS1 | 6009 | − | − | + | − | − | − | Tox− | Patient with diarrhea |
a
+, amplified or present; −, not amplified or not present.
b
Presence or absence of LCT genes (PaLoc) detected with Lok3-Lok1 PCR.
c
Restriction pattern of tcdB fragment B1 and tcdA fragment A3 (type according to the work of Rupnik et al.) (22).
d
Isolates 3126 and 3133 were recovered from the same patient.