TABLE 1.

Primers used for amplification and sequencing of the ankA gene

No. of ticks	First PCR		Nested PCR		Additional primers used for sequencinga
	Primers used	Reference or source	Primers used	Reference or source
15 of 42	U7 + 1R1	32	U8 + 1R7	32	FvL ankU3
			U5 + 1R4	32	FvL ankU1
			U3 + 1R2	32	FvL ankU2
15 of 42	1F + 4R1	32	1F + 1R	32	FvL ank1
			2F1 + 2R1	32	FvL ank2
			3F + 3R	32	FvL ank3
			4F1 + 4R1	32
15 of 42	4F2 + D2	32	4F3 + D1	32
8 of 42	U8 + 1Rmod	32; this study	U8 + 1R7mod	32; this study
			U5mod + 1R4mod	This study
			U3mod + 1R2mod	This study	U3 Seq1
					U3 Seq2
					U3 Seq3
					U3 Seq4
8 of 42	1F + 4R1mod	32; this study	1Fmod2 + 1Rmod	This study
			2F1 mod + 2R1mod	This study
8 of 42	1Fmod2 + D1	This study; 32	3Fmod + D1	32; this study	3Fmod Seq1
					3Fmod Seq2
					3Fmod Seq3
					3Fmod Seq4
					3Fmod Seq5
					3Fmod Seq6
8 of 42	4F2 + D2	32	4F3 + D1	32
1 of 42	U7 + 1R1	32	U8 + 1R7	32	FvL ankU3
			U5 + 1R4	32	FvL ankU1
			U3 + 1R2	32	FvL ankU2
1 of 42	1F + 4R1mod	32; this study	1F + 1R	32
			2F1neu + 4Rneu	This study	2F1neu Seq1
					2F1neu Seq2
					2F1neu Seq3
					2F1neu Seq4
					4F1neu Seq1
					4F1neu Seq2
					4F1neu Seq3
					4F1neu Seq4
					4F1neu Seq5
1 of 42	4F2neu + D1	32; this study

^aPrimers were designed for this study and are listed in Table 2.