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. 2003 Nov;41(11):5033–5040. doi: 10.1128/JCM.41.11.5033-5040.2003

TABLE 2.

Nucleotide sequences of primers used for amplification and sequencing of the ankA gene

Primer Nucleotide sequence
Amplification
    U5mod GCA AGC ACG TGA GGC AGC G
    U3mod GCG TAT GGA TCA CGA GAA AA
    1Fmod2 GTA TTC TGC AGT CTG CTC TTA G
    1Rmod CTT AGT GCT TCA GCG GTC AG
    1R2mod GCG TAG GAA ACT TAG AAC TA
    1R4mod CAT ACT GCA CTG CAC TCA TCC
    1R7mod ATA TCT GCT CCA TAC CAC TG
    2F1mod CAC TGA AGT GGT TGC TGT AA
    2R1mod GAA TAT AAA GCC TCA TGT AAC G
    2F1neu CAA GAG ACA ACT CTG GCT TTT
    3Fmod GCT GCA AGC CGC TCC ATT CT
    4F2neu CAG AAG GAT TAC AGG GAG CA
    4R1mod CTT TGA GGA GCT TCT GGT TG
    4Rneu GGA TCC TGC TTT GCC TTT TC
Sequencing
    FvL ankU1 CAC TCC GCT TCA TAT TGC TA
    FvL ankU2 ACT CCT GAG TCT GTT GTA AA
    FvL ankU3 TAA CAC ATC CTC TTT CAA CC
    FvL ank1 GAA GAA ATT ACA ACT CCT GAA
    FvL ank2 GCA GCT GCT AAT GGT GAC GG
    FvL ank3 TAT CAT CCT TGG GTA GTG G
    U3 Seq1 GCT CAG GTT CCC CAG AAG TA
    U3 Seq2 AAT TTT GGC TAT CAT TAG AAG AT
    U3 Seq3 GAA CAC CCT CAA TCA ACA TC
    U3 Seq4 CAT CTC CAG TGA CAG GTG TA
    2F1neu Seq1 GGA TCT ATG GAT GAA CAA GG
    2F1neu Seq2 ATT AAC AAG TGC CAG GTG TAA
    2F1neu Seq3 CAG TAA TGA TTC TCC GCT TG
    2F1neu Seq4 GTA TCA CCA GAA TGT TTC CT
    3Fmod Seq1 CCA TTT GCT CTT TGC TGA GAA
    3Fmod Seq2 CTG GGG CAA CAG CAT CAG T
    3Fmod Seq3 CAC TGG AGA ATA AAG AAG TAG G
    3Fmod Seq4 ATG CAA CAG TGA AAA AGG GTC
    3Fmod Seq5 CTT TTT GAG AAG TAT CAC TC
    3Fmod Seq6 GCT GAA GGT CTA GGA ACG CC
    4F1neu Seq1 GTC AGC ATA GAT AGA TTC AGG
    4F1neu Seq2 AGA AGA AAT AGA TTC CCA AGT A
    4F1neu Seq3 GCC TAT CTA CGA GGA CAT TA
    4F1neu Seq4 TAG AAG AGC GTG TCC AAG TA
    4F1neu Seq5 TCT TTC TTC TTA ACC ACA GAT A