TABLE 2.
Primers used for amplification and sequencing of the nine loci evaluated for the B. henselae MLST schemea
| Locus | Putative gene product | Product size (bp) | Position on gene (GenBank no.) | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Reference |
|---|---|---|---|---|---|---|
| 16S rDNA | RNA small subunit | 511 | 10-521 (M73229) | AGAGTTTGATCCTGGYTCAG | CTTTACGCCCARTAAWTCCG | 5 |
| batR | Two-component regulator | 487 | 69-555 (AJ300267) | GACCGCAATATTTTGACATC | GCATCCATCAAAGCATCACGACTT | This study |
| eno | Enolase | 472 | 378-849 (AY074768) | AGCAGCAGAGTCATTGTC | CTCAGCCATACCATCTTC | This study |
| ftsZ | Cell division protein | 522 | 1095-1616 (AF061746) | GCCTTCTCATCCTCAACTTC | CTTTGTTTTAAACGCTGCC | This study |
| gltA | Citrate synthase | 379 | 782-1160 (L38987) | GGGGACCAGCTCATGGTGG | AATGCAAAAAGAACAGTAAACA | 30 |
| groEL | Hsp60 chaperone | 405 | 1185-1589 (AF014829) | GTTGATGATGCCTTGAAC | TGGTGTGTCTTTCTTTGG | This study |
| nlpD | Cell surface glycoprotein | 494 | 1061-1554 (AF484425) | GGCGCTGGTATGATACAA | GACATCTGTGCGGAAGAA | This study |
| ribC | Riboflavin synthase | 321 | 1230-1550 (AJ132928) | AGCGAGGATCAAAACAAC | GCTCTTCAACACAATTAACG | This study |
| rpoB | RNA polymerase beta subunit | 471 | 1702-2172 (AF171070) | CGTGACGTACATCCTACA | AACAGCAGCTCCTGAATC | This study |
a
All reference sequences were obtained from B. henselae Houston-1T, with the exception of AJ300267 (batR), which was obtained from Bartonella bacilliformis KC583T. Y = C or T; R = A or G; W = A or T.