FIG. 2.

Reverse line blot hybridization assay for the detection and identification of Entamoeba species and genetic variants. (A) Fecal samples in which uninucleated Entamoeba cysts were found by microscopy; (B) fecal samples in which E. histolytica or E. dispar cysts were found by microscopy and that produced an amplicon after PCR with E. histolytica-specific primers; (C) fecal samples from rural villages in northern Ghana. The oligonucleotide probes were coupled to the membrane in horizontal direction (the numbers on the right refer to the numbers for the oligonucleotide names in Table 2), and the PCR samples were applied in the vertical direction.