TABLE 2.
Phenotypic and genotypic properties of 100 MRSA isolates from the Czech Republic
| Ribotype | PFGE type | No. of isolates | Clonal type | Antibiotic resistanceb (>50% of the isolates) | Presence of aminoglycosidesc
|
Presence of macrolidesc
|
MLST typed | ST | SCCmec type | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aac6′-aph2′′ | aph3′ | ant4′ | ermA | ermB | ermC | msrA | ||||||||
| Collection 1a | ||||||||||||||
| H1 | A1 | 1 | H1:A | O, E, G, K, S, TO, CLI, C, T, R | + | − | + | + | − | + | − | 3-3-1-12-4-4-16 | 247 | IA |
| H2 | A2 | 1 | H2:A | O, E, G, K, S, TO, | + | − | + | + | − | + | − | 3-3-1-12-4-4-16 | 247 | |
| Iberian | CLI, C, T, R | |||||||||||||
| H6 | F1 | 36 | H6:F Czech | O, E, G, K, S, TO, CLI, C, T, R | + | − (7 +) | −(1 +) | + (1 −) | − | + (3 −) | − | 2-3-1-1-4-4-3 | 239 | IIIA |
| F3 | 1 | O, E, G, K, S, TO, CLI, C, T, R | + | − | + | + | − | + | − | |||||
| F5 | 1 | O, E, G, K, S, TO, CLI, C, T, R | + | − | + | + | − | + | − | |||||
| F6 | 2 | O, E, G, K, S, TO, CLI, C, T, R | + | − | + | + | − | + | − | |||||
| H6 | B1 | 1 | H6:B | O, E, G, K, S, TO, CLI, C, T, R | + | − | − | + | − | − | − | 2-3-1-1-4-4-3 | 239 | IIIA |
| H7 | H2 | 1 | H7:H | O, E, CLI, C | + | − | − | − | − | + | − | |||
| H8 | I | 1 | H8:I | O, E, G, K, S, TO, CLI, C | + | + | − | + | − | + | − | 1-4-1-4-46-24-29 | 111 | I |
| Collection 2 | ||||||||||||||
| Hospital 16 | ||||||||||||||
| H1 | B1 | 3 | H1:B Brazilian | O, E, G, K, S, TO, CLI, SXT, C, T, R | + | + | − | + | − | + | − | 2-3-1-1-4-4-3 | 239 | IIIA |
| B5 | 1 | O, E, G, K, S, TO, SXT, C, T, F, CHL, R | + | + | − | + | − | − | − | |||||
| H6 | F1 | 14 | H6:F Czech | O, E, G, K, S, TO, CLI, C, T, R | + (1 −) | − | − (+ 1) | + | − | + (2 −) | − | 2-3-1-1-4-4-3 | 239 | IIIA |
| F2 | 2 | O, E, G, K, S, TO, CLI, C, T, Re | ± | − | − | + | − | + | − | |||||
| F3 | 1 | O, E, G, K, S, TO, CLI, C, T, R | + | − | − | + | − | + | − | |||||
| F5 | 1 | O, E, G, K, S, TO, CLI, C, T, R | + | − | − | + | − | + | − | |||||
| Hospital 20 | ||||||||||||||
| H6 | F1 | 10 | H6:F Czech | O, E, G, K, S, TO, CLI, C, T, R | + | − | −(4 +) | + | − | + (4 −) | − | 2-3-1-1-4-4-3 | 239 | IIIA |
| Hospital 42 | ||||||||||||||
| H2 | A1 | 1 | H2:A Iberian | O, E, G, K, S, TO, CLI, C, T, R | − | − | − | − | − | + | + (1 −) | 3-3-1-12-4-4-16 | 247 | IA |
| A2 | 19 | O, E, G, K, S, TO, CLI, C, T, R | + (1 −) | − | + | + (1 −) | − | − | − | |||||
| H7 | H1 | 3 | H7:H | O, E, C | − | − | − | − | − | + | + (1 −) | 7-6-1-5-8-8-6 | 22 | IV |
Collection 1, isolates collected exclusively from blood samples from 20 hospitals; collection 2, isolates collected from three different hospitals from a variety of clinical sources.
O, oxacillin; C, ciprofloxacin; CLI, clindamycin; E, erythromycin; T, tetracycline; G, gentamicin; K, kanamycin; S, streptomycin; TO, tobramycin; SXT, trimethoprim-sulfamethoxazole; CHL, chloramphenicol; R, rifampin; F, fusidic acid.
+, positive, −, negative; exceptions are indicated in parentheses.
The strains characterized by MLST were the following: CCM 7109 (ST22, SCCmec type IV, PFGE SmaI type H, ribotype HindIII H7), CCM 7110 (ST111, SCCmec type, PFGE SmaI type I, ribotype HindIII H8), CCM 7111 (ST239, SCCmec type IIIA, PFGE SmaI type B, ribotype HindIII H1), CCM 7112 (ST239, SCCmec type IIIA, PFGE SmaI type F, ribotype HindIII H6), CCM 7113 (ST239, SCCmec type IIIA, PFGE SmaI type B, ribotype HindIII H6), CCM 7114 (ST247, SCCmec type IA, PFGE SmaI type A, ribotype HindIII H1), and CCM 7115 (ST247, SCCmec type IA, PFGE SmaI type A, ribotype HindIII H2). CCM refers to the Czech Culture Collection of Microorganisms.
One isolate was susceptible to gentamicin, kanamycin, and tobramycin.