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. 2009 Jan 13;106(3):803–807. doi: 10.1073/pnas.0804270105

Fig. 4.

Fig. 4.

Effects of Mg-ProtoXI in tobacco BY-2 cells. Cells were sampled at the indicated times after dilution, and reagents were added at the time of dilution. (A–C) Changes in Pt/Nu and Mt/Nu ratios of DNA copy number as determined by qPCR and BrdU incorporation (mean ± SD; n = 3). Each sample was pulse-labeled for 30 min with 100 μM BrdU and 10 μM 5-fluorodeoxyuridine. (D–F) Changes in DNA copy numbers in plastid, mitochondrion, and nucleus determined by VIMPCS analysis, expressed as relative copy numbers normalized to the state at the subcultivation (mean ± SD; n = 10). (G) Activity of CDKA-type kinase with histone H1 as substrate and immunoblot analysis with anti-PSTAIRE at the indicated times after dilution. Quantitative data for kinase activity are presented in Fig. S6.