Table 1. Levels of PGEM, kynurenine, and tryptophan in BxPC3 and MiaPaCa2 human pancreatic cancer tumor cell lines.
Results are shown for uninfected (wildtype), vector- (neo) or MUC1-infected BxPC3 cells, and for untransfected (wildtype), control siRNA- (luciferase) or MUC1 siRNA-transfected MiaPaCa2 cells. Average values from n=3 samples in 2 separate experiments are reported. For MiaPaCa2 cells, all data are reported for 48h post siRNA treatment. Similar results were obtained with 72h post treatment. * Represents significant difference between MUC1-expressing and MUC1-depleted cells from wildtype and control cells.
| Pancreatic Cancer Cell lines | Percent MUC1+ cells by flow cytometry | PGEM levels in supernatant
(ngs/ml) |
PGEM levels in lysate
(ngs/ml) |
Tryptophan levels in lysate
(ugs/ml) |
Kynurenine levels in lysate
(ugs/ml) |
|---|---|---|---|---|---|
| BxPC3
(wildtype) |
<0.5 | 255 ± 30 | 1025 ± 112 | 18.3 ± 5.5 | 312 ± 35 |
| BxPC3
(neo) |
<1 | 312 ± 55 | 1242 ± 96 | 15.6 ± 6.8 | 437 ± 52 |
| BxPC3
(MUC1) |
85* | 623 ± 73* | 4037 ± 134* | 6.2 ± 2.6* | 865 ± 105* |
| MiaPaCa
(wildtype) |
95 | 1025 ± 213 | 2456 ± 421 | 29.1 ± 6.3 | 1022 ± 112 |
| MiaPaCa
(luciferase siRNA) |
93
(48h post siRNA) |
1546 ± 178 | 3021 ± 265 | 33.8 ± 12.1 | 1189 ± 216 |
| MiaPaCa
(MUC1siRNA) |
<10*
(48h post siRNA) |
735 ± 98* | 964 ± 123* | 10.3 ± 4.8* | 3045 ± 534* |