Table 2.
Oligonucleotide primers used in this study
| Name | Sequence 5’-3’ | Characteristics |
|---|---|---|
| NEO-RBS | GCGCTGCAGAGGAGGAAAAATATGAATGGACCAATAATAATGACT | Neo 5’ RBS and PstI site |
| NEO-END | GCGCTGCAGCGTTCAAAATGGTATGCGTTTTGA | Neo 3’ PstI site |
| Y154C quik 5’ | TCCTGACCTATGTGTGTGGTAAAGAAACTCCTG | Y154C 5’ quik change |
| Y154C quik 3’ | CAGGAGTTTCTTTACCACACACATAGGTCAGGA | Y154C 3’ quik change |
| prfA-PstI-amplify-+3F | GCGCTGCAGGAACGCTCAAGCAGAAGAATTC | prfA 5’ PstI site |
| prfA-PstI-amplify-+841R | GCGCTGCAGGGAACAACTATCTGTTGCAGCTC | prfA 3’ PstI site |