Table 3.
Time (hours)a | OD600b | NF-L1123 (ΔprfA) | NF-L1124 (prfA) | NF-L924 (prfA E77K) | NF-L943 (prfA G155S) | NF-L1213 (prfA Y154C) |
---|---|---|---|---|---|---|
1 | ~0.03 | 0.5c (26 ± 9.2)d | 1.1 (107 ± 13) | 3.8 (2980 ± 98) | 2.5 (5205 ± 409) | 0.9 (659 ± 0) |
3 | ~0.2 | 0.5 (6.7 ± 0) | 11 (404 ± 8.7) | 36 (7031 ± 84) | 20 (7312 ± 137) | 5.1 (735 ± 3.1) |
5 | ~1.2 | 0.5 (3.8 ± 0.4) | 33 (848 ± 52) | 77 (3764 ± 25) | 10 (4377 ± 184) | 6.9 (1175 ± 61) |
7 | ~2.0 | 1 (2.3 ± 0) | 17 (87 ± 0.4) | 93 (2654 ± 232) | 31 (874 ± 19) | 8.8 (190 ± 0.7) |
Hours growth in broth culture
Approximate bacterial culture optical density at 600 nm
Fold increase in GUS activity observed for growth in BHIC compared to growth in BHI at the same time point.
(Maximum GUS units in BHIC + SD)