Table 3.
Fold change of actA/gus expression in BHIC compared to BHI and maximum GUS units produced during exponential growth
| Time (hours)a | OD600b | NF-L1123 (ΔprfA) | NF-L1124 (prfA) | NF-L924 (prfA E77K) | NF-L943 (prfA G155S) | NF-L1213 (prfA Y154C) |
|---|---|---|---|---|---|---|
| 1 | ~0.03 | 0.5c (26 ± 9.2)d | 1.1 (107 ± 13) | 3.8 (2980 ± 98) | 2.5 (5205 ± 409) | 0.9 (659 ± 0) |
| 3 | ~0.2 | 0.5 (6.7 ± 0) | 11 (404 ± 8.7) | 36 (7031 ± 84) | 20 (7312 ± 137) | 5.1 (735 ± 3.1) |
| 5 | ~1.2 | 0.5 (3.8 ± 0.4) | 33 (848 ± 52) | 77 (3764 ± 25) | 10 (4377 ± 184) | 6.9 (1175 ± 61) |
| 7 | ~2.0 | 1 (2.3 ± 0) | 17 (87 ± 0.4) | 93 (2654 ± 232) | 31 (874 ± 19) | 8.8 (190 ± 0.7) |
a
Hours growth in broth culture
b
Approximate bacterial culture optical density at 600 nm
c
Fold increase in GUS activity observed for growth in BHIC compared to growth in BHI at the same time point.
d
(Maximum GUS units in BHIC + SD)