Early induction of IFN-γ and CXCL9 in allografts is CD8 T cell dependent. A. C57BL/6 mice received syngeneic or complete MHC mismatched A/J cardiac allografts. Groups of 4 iso- (—) and allografts ( - - ) were harvested 24, 48, and 72 hours post-transplant, RNA was isolated from total graft homogenates, and relative real-time PCR was used to measure cytokine and chemokine expression. Data are normalized relative to isograft expression levels at 24 hours post-transplant. (
= p ≤ 0.05) B. Wild-type C57BL/6, B6.CD4−/−, B6.CD8−/− or B6.Rag1−/− mice received syngeneic or A/J cardiac allografts. Groups of 5 grafts were harvested 72 hours post-transplant and intragraft levels of CXCL9 were measured by ELISA. (
= p ≤ 0.0001,
= p ≤ 0.002)