Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 Jan 13.

Published in final edited form as: Am J Transplant. 2008 Jun 18;8(8):1652–1661. doi: 10.1111/j.1600-6143.2008.02302.x

Early CD8 memory T cell alloreactivity enhances recruitment of primed effector T cells to the allograft. Wild-type C57BL/6 mice were treated with control Ig or CD8 depleting antibodies (0.2 mg on days −3, −2, −1, +1). On day 0 mice received A/J cardiac allografts. On day +2, 8 × 10⁶ purified CD90.1 CD4⁺ cells from spleens of wild-type CD90.1 C57BL/6 mice rejecting A/J cardiac allografts on day 7 post-transplant were adoptively transferred and 48 hours later allografts were harvested and graft-infiltrating CD90.1 CD4⁺ T cells were detected using flow cytometry. A. Representative data illustrating the gating strategy used to identify the graft-infiltrating CD4⁺ CD90.1 test population. B. Quantification of the total number of graft-infiltrating CD4⁺ CD90.1 cells (n = 6/group, = p ≤ 0.05). C–D. Relative mRNA quantification of IFN-γ and CXCL9 in grafts harvested from IgG and γ-CD8 treated recipients 4 days after transplantation and 48 hours after adoptive transfer. Data are normalized to a sample randomly chosen from the γ-CD8 treated group (n = 6/group, = p ≤ 0.05).

Inline graphic — Early CD8 memory T cell alloreactivity enhances recruitment of primed effector T cells to the allograft. Wild-type C57BL/6 mice were treated with control Ig or CD8 depleting antibodies (0.2 mg on days −3, −2, −1, +1). On day 0 mice received A/J cardiac allografts. On day +2, 8 × 10⁶ purified CD90.1 CD4⁺ cells from spleens of wild-type CD90.1 C57BL/6 mice rejecting A/J cardiac allografts on day 7 post-transplant were adoptively transferred and 48 hours later allografts were harvested and graft-infiltrating CD90.1 CD4⁺ T cells were detected using flow cytometry. A. Representative data illustrating the gating strategy used to identify the graft-infiltrating CD4⁺ CD90.1 test population. B. Quantification of the total number of graft-infiltrating CD4⁺ CD90.1 cells (n = 6/group, = p ≤ 0.05). C–D. Relative mRNA quantification of IFN-γ and CXCL9 in grafts harvested from IgG and γ-CD8 treated recipients 4 days after transplantation and 48 hours after adoptive transfer. Data are normalized to a sample randomly chosen from the γ-CD8 treated group (n = 6/group, = p ≤ 0.05).