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. 2008 Nov 10;94(1):87–93. doi: 10.3324/haematol.13483

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Figure 4. — XIAP and XAF-1 colocalized in the cytosolic fraction and were co-immunoprecipitated. (A) XIAP and XAF-1 localizations were evaluated by immunoblot analysis after subcellular fractionation of NCI-H929 cells. Enrichment of fractions was verified by expression of PARP1, COX IV and tubulin for nuclear, mitochondrial and cytoplasmic fraction respectively. (B) XIAP and XAF-1 co-immunoprecipitation in NCI-H929 cells. First lane (Input) corresponds to the cell lysate used for immunoprecipitation (IP) and second lane (IP XIAP) corresponds to the lysate obtained after IP.