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. 2008 Dec 4;94(1):78–86. doi: 10.3324/haematol.13426

Figure 2.

Figure 2.

(A) Knockdown of MMSET expression in JIM-3 and H929 human MM cell lines. MMSET was knocked down by transfecting 273 and 490 siRNAs using SLO-permeabilization. MMSET knockdown was assessed at 48h post-transfection by Western blotting analysis. Detection of α-tubulin expression was used to ensure equal loading. (B) Knockdown of MMSET affects proliferation in cells with the t(4;14) translocation. The effect of MMSET on JIM-3 and H929 cells, both of which carry the t(4;14) translocation and RPMI8226 proliferation was assessed by exposing cells to SLO only, SLO with Neg CTL siRNA, 273 siRNA and 490 siRNA. Growth was assessed at 0, 24, 48 and 72 hours by CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. Values represent mean ± SD of three independent experiments. ** represents p<0.05, two way ANOVA test. (C) Knockdown of MMSET affects cell cycle of human MM cells carrying the t(4;14) translocation. The effect of MMSET knockdown on JIM-3, H929 and RPMI8226 cell cycle was assessed by exposing cells to SLO only, SLO with Neg. CTL siRNA and 273 siRNA for 48 hours. Cell cycle profile was evaluated by flow cytometry using propidium iodide. The data is a representation of three independent experiments.